Figure 7.
The effects of caspase inhibitor Z-Val-Ala-Asp-CH2F (Z-VAD.fmk) on the secondary autologous mixed lymphocyte reaction (AMLR) response following nucleohistone (NH) priming. Peripheral blood mononuclear cells (PBMC) were initially stimulated with 50 µg/ml bovine NH for 11 days. T-cell proliferation assays were carried out with these cells stimulated with equivalent numbers of irradiated autologous feeder cells in the presence of 10 µm (closed squares) or 30 µm (closed triangles) Z-VAD, or medium alone (closed circles). Cultures with 15 µm (open squares) or 45 µm (open triangles) dimethylsulphoxide (DMSO) were used as controls for 10 µm and 30 µm Z-VAD, respectively. Each point represents the mean value of triplicate wells (1 × 105 primed-cells and 1 × 105 feeder cells per well), and error bars indicate the standard deviation (SD). *P < 0·001 comparing NH-primed cells cultured in the presence of 30 µm Z-VAD with DMSO controls.