Figure 1.

Activation and migration of splenic T and B cells in response to blood-borne antigen. Sections (400 µm) of spleens from 5C.C7 αβ T-cell receptor (TCR) transgenic mice injected intravenously, 4 hr previously, with phosphate-buffered saline (PBS) alone (a), or with PBS containing 70 µg of cytochrome c protein (cyt c) (b), were double-stained with phycoerythrin (PE)-conjugated MCC 88-103/I-E^k tetramer and fluorescein isothiocyanate (FITC)-conjugated anti-B220 and viewed using a laser-scanning confocal microscope with a 10× objective (panels a and b) or a 60× objective (panel c). Antigen-specific T cells are viewed as red and B cells are green. The T-cell area is marked by ‘T’ and the B-cell area is marked by ‘B’ in panel (a). Similar results were obtained from three PBS- and three cyt c-injected animals.