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. 2002 Dec;107(4):420–425. doi: 10.1046/j.1365-2567.2002.01527.x

Figure 3.

Figure 3

Blood-borne cytochrome c protein (cyt c) induces rapid T-cell activation in the spleen. (A) Spleen cells from 5C.C7 T-cell receptor (TCR) αβ-chain transgenic mice (left panels) and β-chain transgenic mice (right panels), which had been injected intravenously with 70 µg of cyt c 1 hr (a and e), 2.5 hr (b and f), 4 hr (c and g) and 72 hr (d and h) previously, were isolated and stained with antibodies against Vα11, CD4, CD69, B220 and propidium iodide (PI). Histograms represent CD69 expression on T cells (Vα11 positive, B220 and PI negative). The dotted lines represent CD69 expression on T cells from animals injected with phosphate-buffered saline (PBS), 4 hr previously. Similar results were obtained from 20 (0 hr), three (1 hr), four (2.5 hr), eight (4 hr), and six (72 hr) 5C.C7 αβ-transgenic mice and from 19 (0 hr), four (1 hr), four (2.5 hr), six (4 hr), and four (72 hr) 5C.C7 β-transgenic mice. (B) Spleen cells from 5C.C7 TCR β-chain transgenic mice injected intravenously 4 hr previously with PBS alone (a), or with PBS containing 70 µg of cyt c (b), were stained with MCC 88-103/I-Ek tetramer, anti-CD69 and anti-B220 antibodies. PI- and B220-positive cells were excluded from the analysis.