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. 2002 Dec;107(4):420–425. doi: 10.1046/j.1365-2567.2002.01527.x

Figure 5.

Figure 5

Splenic B cells from mice injected with cytochrome c protein (cyt c) stimulate cyt c-specific T cells in vitro. A total of 1 × 105 5C.C7 αβ T-cell receptor (TCR) transgenic lymph node cells were cocultured for 12 hr with 2 × 105 total splenic cells (panels a and b) or with 2 × 105 B220-enriched splenic cells (panels d and e) isolated from B10.BR mice injected with phosphate-buffered saline (PBS) (a and d) or with a single dose of 70 µg of cyt c protein (b and e) 4 hr previously or 5C.C7 αβ TCR transgenic lymph node cells cultured in media alone (c). Cells were stained with antibodies against CD69 [coupled to fluorescein isothiocyanate (FITC)], CD4 [coupled to phycoerythrin (PE)] and B220 (coupled with cy-chrome), to analyse for CD69 expression on CD4-positive T cells. Three experiments showed similar results.