Table 1. Analysis of haematopoiesis in CD11/Hex transgenic mice; absolute numbers and cycling status of myeloid and erythroid progenitor cells in CD11a/Hex transgenic mice.
| Absolute numbers of myeloid progenitor cells × 106/organ | Cycling rates of myeloid progenitor progenitor cells (% in S-Phase) | ||||||
|---|---|---|---|---|---|---|---|
| Nucleated cellularity × 106/organ | CFU-GM | BFU-E | CFU-GEMM | CFU-GM | BFU-E | CFU-GEMM | |
| Femoral bone marrow | |||||||
| Wild-type | 15·0 ± 1 | 16·1 ± 0·9 | 2·0 ± 0·1 | 1·4 ± 0·1 | 15 ± 2 | 13 ± 4 | 14 ± 4 |
| Transgenic | 16·0 ± 1 | 20·0 ± 1·5 | 3·1 ± 0·3 | 1·8 ± 0·2 | 36 ± 7 | 40 ± 7 | 38 ± 7 |
| Fold change | 1·06 | 1·24 | 1·55 | 1·29 | 2·40 | 3·08 | 2·71 |
| P-value | >0·05 | <0·02 | <0·001 | <0·002 | <0·004 | <0·002 | <0·005 |
| Spleen | |||||||
| Wild-type | 138 ± 11 | 10·0 ± 1·1 | 1·7 ± 0·2 | 1·0 ± 0·1 | 2 ± 1 | 2 ± 1 | 1 ± 1 |
| Transgenic | 122 ± 9 | 14·7 ± 2·3 | 3·3 ± 0·7 | 1·8 ± 0·04 | 37 ± 8 | 46 ± 8 | 40 ± 8 |
| Fold change | 0·88 | 1·47 | 1·94 | 1·80 | 18·5 | 23·0 | 40·0 |
| P-value | >0·05 | <0·04 | <0·02 | <0·02 | <0·001 | <0·001 | <0·001 |
Absolute haematopoietic progenitor numbers were quantified by colony formation assays, and progenitor proliferation was measured by thymidine suicide assays. The data represent the mean ± SEM obtained from 10 CD11a/Hex transgenic and 10 wild-type mice analysed individually in two separate experiments.