Table 2. Cytokine profile and the concentrations produced by myelin oligodendrocyte glycoprotein (MOG)-reactive T-cell lines (pg/ml).

Subjects	Antigen	Number of T-cell lines	IL-10	IL-4	TNF-α	IFN-γ
MS	MOG-1	50	362 ± 36	307 ± 64	297 ± 5*	59 ± 10
	MOG-2	43	372 ± 26	185 ± 36*	957 ± 155	37 ± 5
	MOG-3	46	238 ± 34	309 ± 63*	409 ± 89	40 ± 9
	MOG-4	42	299 ± 37	157 ± 44*	453 ± 54*	33 ± 5
NS	MOG-1	48	338 ± 21	219 ± 68	974 ± 113	31 ± 6*
	MOG-2	50	294 ± 15	420 ± 100	952 ± 102	23 ± 5
	MOG-3	41	341 ± 20	141 ± 43	518 ± 71	16 ± 4
	MOG-4	46	397 ± 23	436 ± 80	1346 ± 11	37 ± 7

MOG-reactive T-cell lines were challenged with the corresponding MOG fragments, and the supernatants were collected after 48 hr. The absolute cytokine concentrations were measured by enzyme-linked immunosorbent assay (ELISA). The detection limits for all cytokines studied were between 15 and 35 pg/ml.

^*Significant differences in the cytokine production between multiple sclerosis (MS)-derived T-cell lines and normal subjects (NS)-derived T-cell lines specific for the same MOG fragments.

IFN-γ, interferon-γ; IL, interleukin; TNF-α, tumour necrosis factor-α.