Figure 5.

T cells were activated for 4 days with ConA (5 μg/ml) before being washed extensively and cultured in reduced serum conditions. Cells were then either preincubated with dicumarol (75 μm) or not for 20 min before addition of IGF-1 and anti-Fas antibody. Viability was assessed by trypan blue exclusion after 6 hr. Data are presented as mean viable cell numbers from several independent experiments and vertical bars represent standard deviations from the mean. Western blotting with anti-phospho-c-Jun and anti-JNK antibodies were used to assess c-Jun phosphorylation and loading, respectively.