Table 1.
Analysis of correlation between cytokine gene expression in thoracic lymph nodes and magnitude of airway eosinophilia
| Cytokine* | Correlation with Eosinophil number in BALF† | Statistical significance | |
|---|---|---|---|
| Wild-type | IFN-γ | −0·65 | P = 0·022 |
| IL-4 | 0·55 | P = 0·063 | |
| IL-5 | 0·71 | P = 0·01 | |
| IL-10 | 0·36 | P = 0·25 | |
| IL-25 | 0·34 | P = 0·29 | |
| IL-4/IFN-γ | 0·73 | P = 0·007 | |
| TCRγδ KO | IFN-γ | −0·29 | P = 0·37 |
| IL-4 | 0·49 | P = 0·11 | |
| IL-5 | 0·45 | P = 0·14 | |
| IL-10 | 0·028 | P = 0·93 | |
| IL-25 | 0·19 | P = 0·56 | |
| IL-4/IFN-γ | 0·49 | P = 0·11 |
*
Cytokine expression was calculated as a ratio versus GAPDH or as an IL-4/IFN-γ ratio.
†
Spearman rank correlation coefficient.
Statistical significant correlations are indicated by italics (n = 12).The number of eosinophils in BALF was determined 18 hr after the last of three repeated aerosol exposures of OVA in presensitized C57BL/6 wild-type or TCRγδ knockout mice. Immediately after performing BAL, thoracic lymph nodes were removed for semiquantitative analysis of cytokine mRNA.