Table 2.

Analysis of IFN-γ and IL-4 gene expression in the spleen of wild-type and TCRγδ knockout mice

Treatment/strain	IFN-γ/GAPDH	IL-4/GAPDH	IL-4/IFN-γ	Statistical significance
Non immunized wild-type	0·35 ± 0·08	0·29 ± 0·08	0·82 ± 0·13	ns
Immunized wild-type	0·36 ± 0·06	0·30 ± 0·06	0·83 ± 0·17
Immunized TCRγδ KO	0·38 ± 0·07	0·36 ± 0·02	0·97 ± 0·16	ns

Statistical comparisons versus immunized wild-type mice (n = 5 in each group). Mean values ± standard deviations are indicated. ns = non-significant differences for all ratios.

Spleens were removed from OVA-immunized mice 28 days after the first injection. For comparison, data are also shown from non-immunized wild-type mice. The ratio versus the housekeeping gene GAPDH and the IL-4/IFN-γ ratio are shown.