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. 2003 Jan;108(1):32–41. doi: 10.1046/j.1365-2567.2003.01562.x

Figure 2.

Figure 2

Expression of MUC1 in activated T cells. (a) Resting and phytohaemagglutinin (PHA)-activated T cells were stained using different MUC1-specific monoclonal antibodies (mAbs) followed by fluorescein isothiocyanate (FITC)-labelled rabbit anti-mouse immunoglobulin. (b) Unstimulated peripheral blood mononuclear cells (PBMC) (day 0) or PBMC stimulated with anti-CD3 mAb for the indicated periods of time were stained with anti-CD3–FITC, in combination with anti-CD69–PE, HMFG1–biotin or 12C10–biotin, and analysed by flow cytometry. Numbers indicate the percentage of gated cells in the quadrant.