Figure 4.

Analysis by confocal microscopy of MUC1 on activated T cells. (a) Activated T cells were stained for MUC1 (green) and then counterstained for actin (red). The image shown in (a) represents a projection of eight images acquired as 0·5-µm-thick scanned sections, four of which are shown in b–e. Magnification is 100×. (b) Activated T cells adherent to fibronectin-treated slides were treated with regulated on activation, normal, T-cell expressed, and secreted (RANTES) chemokine prior to staining for MUC1 (green; thick arrows). Cells were then permeabilized and stained for spectrin (red; thin arrowheads), a marker for uropods. The magnification of image a is 40×. The magnification of images b–d is 100×. These confocal microscopy images are projections of 16 stacked sections through the cells.