Figure 5.

Detection by reverse transcription–polymerase chain reaction (RT–PCR) of MUC1 transcript in activated T cells. (a) Human T cells were activated in vitro by anti-CD3 antibody. Total RNA was extracted and RT–PCR performed on the indicated days (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4). MUC1 transcript (446 bp) was identified after at least 24 hr. Human β-actin was included as a positive control, and a molecular weight marker is present in lane 1. (b) Semiquantitative RT–PCR for MUC1 in activated T cells and autologous breast cancer. Total RNA was extracted from autologous breast cancer cells and from purified human T cells following 4 days of in vitro activation using anti-CD3 antibody. cDNA was synthesized and then used as the template for RT–PCR at the dilutions shown. Human β-actin was included as a positive control.