Northern blot analysis of resting and activated human T cells. (a) Resting T cells (D0) or T cells activated by anti-CD3 antibody for the indicated periods of time (D0, day 0; D1, day 1; D2, day 2; D3, day 3; D4, day 4) were analysed by Northern blot analysis for expression of MUC1. MUC1* represents the same blot with increased sensitivity. (b) The O-glycosylation enzymes C2GnT1, C2GnT2 and C2GnT3 from resting T cells (D0) or T cells activated by anti-CD3 antibody for the indicated time-periods were analysed for expression by Northern blot analysis. The cell lines T47D, MTSV1-7 and HPAF were included as positive controls.