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. 2003 Feb;108(2):230–237. doi: 10.1046/j.1365-2567.2003.01571.x

Figure 4.

Figure 4

Regulation by cytokines of splenic cells IFN-γ production in C57BL/6 IL-12 knockout and wild-type C57BL/6 mice infected with T. cruzi. Cultures were performed on days 7 and 14 after infection and stimulated with parasite-antigen, T-Ag. At the beginning of the cultures the neutralizing anti-cytokine mAbs, anti-IL-10, anti-IL-4 and anti-TNF-α, were added; GL.113 was used as isotype control mAb. The supernatants were harvested after 72 hr and IFN-γ was measured by enzyme-linked immunosorbent assay. Results are expressed as the arithmetic means from duplicate cultures ± SD. *P < 0·05. The results are representative of three experiments.