Figure 5.

Lysis of aMCMV [Smith's strain murine cytomegalovirus (MCMV), passaged three or four times through the rat fibroblast monolayer]-infected and -uninfected fibroblast monolayers by interleukin-2 (IL-2)-induced blasts developed from γδ T⁺ and γδ T⁻ draining popliteal lymph node (PLNi)-derived lymphocytes. Fifty thousand cells of γδ T⁺ and γδ T⁻ cell fractions were cultured on syngeneic fibroblast monolayers with 100 U/ml human recombinant IL-2 (hrIL-2). Five days later, when clear blasts developed, the cells were harvested, washed twice, and 50 000 cells/well were plated on a ⁵¹Cr-labelled monolayer, untreated or infected 2 hr previously with 1000 plaque-forming units (PFU) of aMCMV with or without 100 U/ml hrIL-2. The percentage of specific ⁵¹Cr release was counted 3 and 6 days after passage. Results represent the mean value ± SD from three independent experiments. *Statistically significant difference (P < 0.01).