Table 1.
Inhibition of plaque development in syngeneic monolayer by different types of rat lymph node-derived cell populations
| No of plaques (Mean ± SD) | PFU/ml in culture fluid | |||||
|---|---|---|---|---|---|---|
| Type of cells* | Days after infection† | Day 4 | Day 7 | Day 8 | Day 10 | Day 12 |
| 1. Infected monolayer only | 47·0 ± 3·7 | > 200 | Confluent | Confluent | 64000 | |
| 2. PLNc, γδ T+ | 2 | 7·0 ± 2·6 | 19·7 ± 2·5 | > 20d | Confluent | 68000 |
| 3. PLNi, γδ T+ | 2 | 2·5 ± 1·3 | 9·3 ± 1·5 | 9·3 ± 1·5 | 9·3 ± 1·5‡ | 3000 |
| 4. PLNi, γδ T− | 2 | 8·3 ± 0·96 | 51·0 ± 6·6 | Confluent | Confluent | 52000 |
| 5. PLNi, CD8+ | 6 | 1·3 ± 0·96 | 0·3 ± 0·5 | 0·3 ± 0·5 | 0 | 0 |
| 6. PLNi, γδ T− | 6 | 4·5 ± 1·3 | 4·0 ± 0·8 | 3·3 ± 1·0 | 3·3 ± 1·0§ | 0 |
| 7. PLNi, γδ T+ | 6 | 0 | 2·3 ± 1·9 | 2·3 ± 1·9 | 2·3 ± 1·9§ | 0 |
| 8. PLNi, γδ T+ (NKR-PI depleted) | 6 | 4·8 ± 1·7 | 4·3 ± 1·0 | 4·3 ± 0·96 | 1·8 ± 0·9 | 0 |
| 9. PLNi, γδ T+(NKR-PI depleted)anti-IFN-γ | 6 | 29·3 ± 4·6 | > 200 | Confluent | Confluent | 64000 |
A total of 50 000 γδ T+ or γδ T− cell fractions, as well as CD8+ cells, were plated on syngeneic fibroblast monolayers (10 000 fibroblasts/well) preinfected 2 hr previously with 50 plaque-forming units (PFU) of aMCMV [Smith's strain murine cytomegalovirus (MCMV), passaged three or four times through the rat fibroblast monolayer] in 0·1 ml of Dulbecco's modified Eagle's minimal essential medium (DMEM); in rows 8 and 9 the negative selected γδ T cells depleted from NKR-P1+ were used. PLNc, contralateral popliteal lymph nodes; PLNi, draining popliteal lymph nodes.
DA rats were injected with 10 000 PFU of aMCMV into the footpad; PLN cells were derived either day 2 or day 6 after inoculation with aMCMV.
Plaques were dramatically enlarged in size.
Plaques were arrested.
IFN-γ, interferon-γ.