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Freshly isolated PBMo and BrMMφ as well as IL-4-stimulated BrMMφ were analysed (a)for mRNA expression of DC-SIGN (upper panel) and GAPDH (lower panel) by RT-PCR and (b) for protein expression of DC-SIGN by FACScan. In FACScan analysis, specific staining (filled area) and negative control staining (open area) were shown in each panel. Note that fresh BrMMφ, but not PBMo, expresses the DC-SIGN gene and protein and its expression was up-regulated after IL-4 stimulation.
