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. 2003 Feb;108(2):152–157. doi: 10.1046/j.1365-2567.2003.01578.x

Figure 3.

Figure 3

Costimulation through ICAM-1 causes down-regulation of p27kip1. (a) T cells were left non-stimulated (lane 1), stimulated with anti-CD3 alone (lane 2), anti-CD3 + anti-ICAM-1 (lane 3), anti-CD3 + anti-LFA-1 (lane 4), or anti-CD3 + anti-CD28 (lane 5) for 5 days. Whole cell lysates were resolved on SDS–PAGE, blotted, and probed to determine the relative level of p27kip1 protein (upper panel). The blot was stripped and reprobed for actin as a loading control (lower panel). (b)Densitometric ratio of p27kip1/actin on the same blot. Representative of four experiments.