Figure 2.
IFN-γ production, sFAS-L production, and antitumour activity of various T-cell subsets of PBMC. The indicated T-cell subsets were purified by cell sorting, and 2 × 105 cells from each subset were stimulated with IL-2, -12 and -15 in 96-well flat-bottom plates for 96 hr, and IFN-γ levels (a) and sFAS-L levels (b) in culture supernatants were determined by ELISA after culturing for 48 hr. The cytotoxicities of the cultured subsets against K562 cells (c) (E/T ratio was 10/1) and against Raji cells (d) (E/T ratio was 10/1) were also measured after culturing for 96 hr. All data represent the means ± SE from four independent experiments.