Figure 1.

Recruitment of CD11c⁺ F4/80⁺ CD11b⁻ (AM), CD11c⁻ F4/80⁺ CD11b⁺ (IM) and CD11c⁺ F4/80⁻ CD11b⁻ (DC) to the lungs of mice shortly after i.n. delivery of BCG. Cells harvested from the BAL and from digested lung tissue 6–96 hr after i.n. delivery of BCG (10⁶ CFU) were stained with antibodies as follows: FITC- or PE-conjugated CD11c, F4/80 and CD11b. Cells expressing CD11c⁺ F4/80⁺ CD11b⁻ (AM) or CD11c⁻ F4/80⁺ CD11b⁺ (IM) or CD11c⁺ F4/80⁻ CD11b⁻ (DC) were analysed by FACScan flow cytometry. Rat IgG2a, rat IgG2b isotypes and hamster IgG were used as controls. The number of cells present in the three populations was calculated as: percentage of each subset as determined by FACS analysis × number of cells counted in the BAL or lung digest, and was expressed as the mean ± SEM. Values of P < 0·05 (*) were considered significant. The results shown are representative of three separate experiments.