Table 1.
Adoptive transfer experiments with ITGBko and ICAM1ko mice. Percentages of donor CFSE+ cells within the total TCR+ cells from spleen, lymph nodes and bone marrow of adoptively transferred mice
| Donor | Recipient | Spleen | Lymph node | Bone marrow | Spleen: lymph node ratio | Spleen: bone marrow ratio |
|---|---|---|---|---|---|---|
| (a) ITBG2ko mouse experiments | ||||||
| B6 | B6 (n = 10) | 3·64 (1·35) | 4·80 (1·79) | 1·75 (0·73) | 1·31†† | 0·53 |
| ITBG2ko | ITBG2ko (n = 10) | 3·50 (1·32) | 3·90 (1·52) | 1·71 (0·77) | 1·11** | 0·48 |
| B6 | ITBG2ko (n = 10) | 3·95 (0·75) | 5·80 (1·55) | 1·48 (0·60) | 1·47††† | 0·37*, † |
| ITBG2ko | B6 (n = 10) | 3·61 (1·49) | 3·54 (2·03) | 2·07 (0·92) | 0·94** | 0·56 |
| (b) ICAM1ko mouse experiments | ||||||
| B6 | B6 (n = 10) | 3·68 (1·30) | 4·63 (1·41) | 2·20 (0·98) | 1·28** | 0·61 |
| ICAM1ko | ICAM1ko (n = 10) | 3·15 (1·20) | 3·28 (1·41) | 2·08 (0·79) | 1·02†† | 0·67 |
| B6 | ICAM1ko (n = 10) | 3·26 (2·51) | 2·96 (2·52) | 1·73 (1·03) | 0·87***,†† | 0·62 |
| ICAM1ko | B6 (n = 10) | 2·88 (0·77) | 3·94 (1·11) | 1·96 (0·60) | 1·37††† | 0·69 |
Spleen and lymph node cells were purified from donor mice, labelled with CFSE and injected intraperitoneally into recipient mice (35–37 million cells per mouse). After 7–19 days from adoptive transfer, spleen, lymph node and bone marrow cells were purified from recipient mice and analysed as in Fig. 2. The average percentages and standard deviations of donor CFSE+ cells within the total TCR+ cells from spleen, lymph nodes and bone marrow of adoptively transferred mice are indicated. The average ratios between the percentages of lymph node and spleen CFSE+ T cells and that between the percentages of bone marrow and spleen CFSE+ T cells are also indicated. (a) Homing of ITGB2ko and B6 mice derived T cells into spleen, lymph nodes and bone marrow of either ITGB2ko or B6 mice (summary of six experiments; total numbers of recipient mice for each group indicated in parentheses). Statistical analysis was performed by Students' t-test. The P-value was calculated for each series of data either versus the B6 donor–B6 recipient group (***P ≤ 0·001, **P ≤ 0·01, *P ≤ 0·05) or versus the ITGB2ko donor–ITGB2ko recipient group (†††P ≤ 0·001, ††P ≤ 0·01, †P ≤ 0·05). (b). Homing of ICAM1ko and B6 mice derived T cells into spleen, lymph nodes and bone marrow of either ICAM1ko or B6 mice (summary of three experiments; total numbers of recipient mice for each group indicated in parentheses). Statistical analysis was performed as in (a). The P-value was calculated for each series of data either versus the B6 donor–B6 recipient group (***P = 0·001, **P = 0·01, *P = 0·05) or versus the ICAM1ko donor-ICAM1ko recipient group (†††P ≤ 0·001, ††P ≤ 0·01,†P ≤ 0·05).