Table 2.
Surface marker expression on different monocyte populations
Marker | Initial | NAD | BND | MIG |
CD11a | 312 ± 138 | 301 ± 154‡ | 273 ± 150 | 362 ± 187*† |
CD33 | 138 ± 63 | 130 ± 49‡ | 118 ± 49 | 148 ± 70*† |
CD45RA | 24 ± 15 | 25 ± 11 | 27 ± 11 | 46 ± 47 |
CD45RB | 397 ± 149 | 367 ± 135‡ | 326 ± 134 | 375 ± 154† |
CD45RO | 64 ± 35 | 80 ± 44 | 79 ± 50 | 111 ± 57*† |
CD49d | 56 ± 10 | 53 ± 10 | 53 ± 12 | 65 ± 28 |
CD54 | 41 ± 32 | 36 ± 12 | 35 ± 15 | 58 ± 33*† |
CD62L | 22 ± 24 | 30 ± 24 | 29 ± 26 | 44 ± 33† |
CD86 | 26 ± 19 | 36 ± 30 | 66 ± 103 | 26 ± 14 |
HLA-DR | 174 ± 83 | 186 ± 89 | 207 ± 104 | 326 ± 254*† |
Data shown are fluorescence intensities (means ± SD) of the initial population and the nonadherent (NAD), bound (BND) and migrated (MIG) monocytes. In all 14 experiments performed, the migration period was 30 minutes; the endothelium was not pretreated. *Statistical significance (P < 0.05) MIG compared with NAD. †Statistical significance (P < 0.05) MIG compared with BND. ‡Statistical significance (P < 0.05) NAD compared with BND.