Table 1.
In situ hybridization flow cytometry assay (ISH-FC): normal peripheral blood leucocytes, mean and SEM of 12 experiments
| Control probes | Test probes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Negative | CD32 | CD35 | TNF | IFN | CD20 | CD21 | CD22 | CD80 | CD86 | IIα | II B | |
| Lymphocytes | ||||||||||||
| Mean | 10 | 10·4 | 12 | 11·4 | 11·2 | 10 | 9·4 | 10·6 | 14·2 | 9 | 11·4 | 11·6 |
| SEM | 1·6 | 1·8 | 2·5 | 3·1 | 2·3 | 1·6 | 1·8 | 1·6 | 1·8 | 1·7 | 2·6 | 3 |
| Monocytes | ||||||||||||
| Mean | 10·5 | 13·5 | 14·3 | 14 | 13·9 | 12·8 | 11·6 | 13·9 | 15·7 | 9·6 | 14 | 14·4 |
| SEM | 1 | 2·2 | 2·3 | 3·4 | 2·7 | 1·4 | 1·8 | 2·1 | 2 | 2 | 2·7 | 3·1 |
| Neutrophils | ||||||||||||
| Mean | 16·8 | 31·6 | 36·9 | 32·8 | 34 | 38·5 | 31·5 | 33·9 | 34·5 | 31·3 | 38·7 | 33·5 |
| SEM | 2 | 3·5 | 4·1 | 6·5 | 5 | 3·6 | 4·2 | 3 | 4·5 | 3 | 4 | 4·1 |
This table shows detection of mRNA specific for various CD antigens using DIG-labelled oligonucleotide probes. Results are expressed as the mean fluorescence intensity of binding (MFI) using normal human peripheral blood leucocytes obtained from 12 different normal donors. The three main leucocyte populations were analysed separately: Region 1 = lymphocytes, R2 = monocytes and R3 = neutrophils.