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. 2003 Mar;108(3):391–400. doi: 10.1046/j.1365-2567.2003.01573.x

Figure 3.

Figure 3

The IL-12p70 inhibitory activity contained in FCM is different from IL-10. (a) Dose–response inhibition of IL-12p70 production by splenic derived DC stimulated with DS-2 obtained in the presence of IL-10, TGF-β1, IFN-β and VEGF. (b) The inhibitory effect of IL-10 on the production of IL-12p70 by day 12 splenic derived DC is efficiently counteracted by a neutralizing anti-IL-10 antibody. (c) The inhibitory effect of thymic FCM on the production of IL-12p70 by day 12 splenic derived DC is not suppressed by a neutralizing anti-IL-10 antibody. Day 12 splenic derived DC were cultured for 24 hr in the presence of increased concentrations of the different inhibitory molecules and then stimulated with DS-2 for further 24 hr of incubation. Anti-IL-10 monoclonal antibody (5 μg/ml) or the corresponding isotype (IgG1; 5 μg/ml) were added to the cultures in (b) and (c) during the first 24 hr of incubation at the same time as IL-10 and FCM. Splenic DC cultures were then stimulated with DS-2 for a further 24 hr.