Figure 6.
Phenotype analysis of splenic derived DC cultured in the absence or the presence of IL-10 and thymic FCM. Day 12 splenic DC were incubated for 24 hr with or without IL-10 (10 ng/ml) or thymic FCM (20%) and then stimulated with DS-1 and PSB-2 (a) or DS-2 (b) for a further 24 hr. The cells were analysed by flow cytometry for the surface expression of the indicated antigens. Dotted line represents negative control; thick bold line represents positive control; a dashed line represents incubation with IL-10 and the bold line represents incubation with FCM.