Table 1.

Putative NF-κB binding sites present within the first 4000 bp of the CRP promoter

κB-like sites centred at position	Nucleotide sequences*
−3920	−3927 AGTAGAGGTTTTCTGTGAGGG −3907
−3906	−3920 GTTTTCTGTGAGGGCTCCATGCCTG −3896
−3834	−3845 CTAGACAGAAACTCCCAAAGC −3825
−3290	−3300 GTGCTTAGAAATTTCTTAAGCAC −3278
−2955	−2966 ATTTTTAGGAATCTTTATAGCAG −2944
−2652	−2662 ATGGGGGAAACCGCTCCTATG −2642
−1700	−1708 ACAGGGAGAGCTCATCCCTTTTAATAG −1682
−1572	−1581 AAGAGGAGAGTATTTCTCCTTTA −1559
−1135	−1145 GATACGGTGGTTCTTTTCTGATT −1123
−966	−975 TGTGGAGGGATTACTTGAATC −955
−780	−791 ATGTCTAGAGAGTTCTTAATAAG −769
−643	−653 ACTCTGGGGACTGTTGTGGGGTG −631
−222	−236 TGGAGCCCTGAGAGATTTCTT −216
κB consensus	CCAAGGGGACTTTCCATG

^*The sequences of the coding strands of 13 possible κB sites on the CRP promoter, that closely match the κB consensus sequence, are shown. These oligonucleotides were used in the EMSAs shown in Fig. 7. The κB-site-like sequences are in bold.