Table 2.

P5-coupled ovalbumin (P5-OVA) down-regulates the cytokine-producing capability of spleen cells from normal CBA mice

	Cytokine levels* in supernatants of anti-CD3-stimulated spleen cells from normal CBA mice with or without P5-OVA
CBA (n = 3)	IFN-γ (pg/ml)	IL-2 (pg/ml)	IL-10 (pg/ml)	IL-4 (pg/ml)
Anti-CD3 (1·25 µg/ml)	19565 ± 160†	2296 ± 64†	2377 ± 541‡	422 ± 100
Anti-CD3+P5-OVA (8 nm)	2798 ± 788†	<12†	215 ± 15‡	251 ± 37
Con A (5 µg/ml)	17477 ± 2078†	1644 ± 387†	2132 ± 212‡	457 ± 29
Con A+P5-OVA (8 nm)	3856 ± 923†	592 ± 171†	344 ± 214‡	292 ± 56
Control	<12	<12	<12	<12

^*The levels of interferon-γ (IFN-γ), interleukin (IL)-2, IL-10 and IL-4 were determined in the supernatants of cell cultures recovered 48 hr poststimulation, as described in the Materials and methods. The data represent mean values ± standard deviation (SD) for three mice and are representative of four independent experiments.

^†A highly significant difference in the IFN-γ and IL-2 levels (P < 0001) was observed between cultures with or without P5-OVA after stimulation with anti-CD3 and concanavalin A (Con A).

^‡A statistically significant difference was observed in the IL-10 levels (P < 0·05) between cultures with or without P5-OVA after stimulation with anti-CD3. The results have been rounded up or down, as appropriate, to the nearest significance figure.