Table 1.
Co-attachment of antigen and anti-CD40 is essential for the adjuvant effect
| Titre determined against | |||
|---|---|---|---|
| Immunization | Avidin | Rat IgG2a | |
| Avidin | 1·23 (0·39) | 1 (0) | |
| Avidin + biot 1C10 | 4·66 (0·13) | P < 10−6 | 4·51 (0·44) P < 10−6 |
| Avidin + biot GL117 | 2·2 (0·59) | NS | 2·1 (0·26) NS |
| Avidin + streptavidin + biot 1C10 | 1·45 (0·89) | NS | 4·53 (0·24) P < 10−6 |
| Avidin + unbiotinylated 1C10 | 1·59 (1·34) | NS | 4·53 (0·24) P < 10−6 |
Antibody responses of mice against avidin and rat IgG2a 14 days after a single immunization with anti-CD40 and avidin in the combinations shown. Immune responses where antigen is attached to a CD40 binding moiety are shown in bold. Significantly enhanced responses is indicated by P < 10−6.