Figure 1.

Up-regulation of A-to-I RNA editing in mouse thymus (a) and spleen (b) during acute systemic inflammation. Spleens and thymuses were harvested (n = 6) at the indicated time-points after endotoxin-challenge and whole cell extracts were examined for editing activity. Editing activity was determined by measuring A-to-I conversion of dsRNA on TLC. The percentage of inosines was estimated in comparison with adenosines and plotted against the duration of endotoxin stimulation (c). pI, 5′-monophosphate inosine; pA, 5′-monophosphate adenosine. The ratio between pI and pA represents the percentage of adenosine that is converted to inosine.