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. 2003 May;109(1):15–23. doi: 10.1046/j.1365-2567.2003.01598.x

Figure 4.

Figure 4

Induction of ADAR1 by inflammatory mediators (a) and pathogens (b) in vitro. (a) Cytotoxic T cells (CTLL-2) and macrophages (MH-S) were stimulated with IFN-γ (1000 U/ml) or TNF-α (10 ng/ml). In both cell types RT-PCR analysis indicated a marked increase in ADAR1 after 4 hr of stimulation. (b) Macrophages (5 × 106 MH-S cells) were stimulated with live E. coli (104−109 CFU) or adenovirus (0·01 and 0·1 MOI) for 2 hr. ADAR1 was up-regulated by both pathogens. Each gel represents a single experiment. Similar observations were uniformly seen in all experiments (n = 3). The primers for RT-PCR cover exons 5–8. Two RT-PCR products were seen in all cases because of alternative splicing in exon 7 (AF291050 and AF291876).