Table 2.

cjTREC levels in the sorted T cells of DP-High and DP-Low group

				cjTRECs (copies/105 T cells)
Group	No.	% of DP T cells in the PBL	% of naive subset in the sorted T cellsA	Number of copiesB	Mean ±SD	The mean copy number of cjTRECs in each naive T cells†
DP-High	2	17.23	6.33	2 778	4 362 ± 3 139*	0.439
	9	14.10	8.15	2 330		0.286
	12	10.10	12.33	7 978		0.647
DP-Low	3	3.10	16.38	17 098	22 722 ± 4 928	1.044
	5	0.70	23.06	26 283		1.140
	6	1.22	19.70	24 785		1.258

The T cells were sorted out by deletion of CD14⁺, CD16⁺, and CD20⁺ lymphocytes from the PBMC. Sorted T cells (1×10⁶) were used for preparation of genomic DNA and a part of them was stained with CD45RA-FITC and CD62L-PE. The naive T cell subset was defined by CD45RA⁺CD62L⁺ phenotype.

^*Indicates significant difference (P < 0.006) in TREC levels between DP-High and DP-Low group (Student's t test).

^†Firstly, the number of naive subset in the 10⁵ T cells was calculated by the percentage^A of naive subset in the sorted T cells and then this number was compared with cjTREC levels^B in the 10⁵ T cells measured in this study. If all naive T cells in the periphery are RTE, this value can be maximally calculated at approximately 1.26 [20].