Figure 3.

Induction of NO in macrophages by cytofectin complexes of synthetic nucleic acids. J774 cells were treated for 48 hr with 0·4–10 µg/ml of ds poly(dG)·poly(dC), poly(dA)·poly(dT), or poly(dI)·poly(dC) with or without Fugene 6. In complexes with the cytofectin agent, all three polymers induced NO, while these DNAs alone were inactive. NO levels in cells cultured with medium alone and Fugene 6 alone were < 2 µm. Each value represents a mean of triplicates. Error bars depict the standard deviations. The data are representative of at least three separate experiments.