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. 2003 Jun;109(2):255–262. doi: 10.1046/j.1365-2567.2003.01653.x

Figure 7.

Figure 7

Confocal microscopy analysis of intracellular distribution of CT DNA. J774 cells were simultaneously incubated with 1 µg/ml of EMA-labelled CT DNA in complexes with Fugene 6 (CT DNA) and 0·5 mg/ml Oregon green-labelled dextran (Dextran) at 37° for 12 hr. Internalized CT DNA (red-coloured) and dextran (green-coloured) were examined by confocal microscopy. CT DNA can be seen mostly co-localized (yellow-coloured) with dextran (Merged), indicating a fluid phase endocytosis pathway for DNA uptake (bar = 10 µm).