Table 2.
Continuous in vivo exposure to ES-62 reduces the recovery of splenic B cells following culture with (Fab′)2 fragments of anti-IgM ex vivo*
| ES-62 release rate | ||
|---|---|---|
| Cell type | 0·5 µg/hr (%) | 0·2 µg/hr (%) |
| Total splenocyte recovery | 58 | 39 |
| All B220+ B cells | 6·3 | 53 |
| B220+PNA+ GC B cells | 32 | 30 |
| All non-GC B220+ B cells | 4·6 | 54 |
*
The recovery of cells in control (PBS-exposed mice) cultures were normalized as 100% and the number of B cells recovered in the ES-62-treated cultures were calculated as a percentage of this. These data are the recoveries calculated from the experiment presented in Fig. 5(c) in which the cells were initially seeded at equivalent cell densities prior to ex vivo culture and this profile of reduced splenocyte recoveries correlating with reduced DNA synthesis was found in all experiments.