Figure 5.

Effect of dexamethasone and actinomycin D on IL-5 mRNA stability. PBMCs were incubated with PMA (50 nm) + PHA (5 μg/ml) for 2 hr then dexamethasone (1 μm) and/or actinomycin D (10 μg/ml) were added and the cells were harvested at the time-points indicated for semi-quantitative RT-PCR analysis of IL-5 and GAPDH. (a) Agarose gels representative of five such experiments are shown. (b) Data were normalized to GAPDH and expressed as a percentage of the response after 2 hr of stimulation. Data from five independent experiments are expressed as means ± SEM.