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Stimulation of MMP-1 secretion and mRNA expression by LDL-IC. U937 cells were incubated with or without 150 µg/ml of LDL-IC for 24 hr. After the incubation, the conditioned medium was collected for quantitative analysis of secreted MMP-1 by ELISA (a) while the total RNA was isolated for Northern analysis of MMP-1 mRNA (b). The data are representatives of three experiments with similar results.
