Figure 4.

LDL-IC stimulate MMP-1 promoter activity in cells transfected with Construct 1. U937 cells were transiently transfected with MMP-1 promoter-reporter constructs and β-galactosidase vector for 24 hr, and then stimulated without (control) or with 150 µg/ml of LDL-IC or 100 nm PMA for 24 hr. The cellular luciferase and β-galactosidase activities were measured and the ratios were calculated for correction of the transfection efficiency. Data presented are mean ± SEM of three experiments run in triplicate.