Figure 6.

LDL-IC stimulate transcription factor AP-1 and Ets activities. Nuclear proteins of U937 cells treated without (C, control) or with 150 µg/ml of LDL-IC (IC) were extracted and incubated with radiolabelled oligonucleotide probes containing AP-1, Ets, or CREB motifs. The binding of transcription factor in the nuclear extracts to their corresponding probe was detected by the EMSA as described under Materials and Methods. FP, free probes. SP, shifted probes. The blot presented is representative of three blots with similar results.