Expression of Bcl family proteins in T cells cultured with LNS cells. Fresh T cells or T cells cultured with LNS cells or with a recombinant cytokine (IL-4, IL-6, and IL-7, 10 ng/ml) in the absence of supporting cells and cytokines (T alone) for 48 hr, were collected and analysed for their expression of Bcl-2 and Bcl-XL by immunoblotting as described in Materials and Methods. In the right panel, T cells were cultured with IFN-β (100 U/ml) for 24 hr with or without a neutralizing antibody and analysed for Bcl-XL expression.