Figure 4.

IL-8-producing activities in human PBMC by various synthetic disaccharide and monosaccharide lipid A analogues. Cells were stimulated with the indicated doses of each test specimen in RPMI-1640 supplemented with 10% FBS at 37° for 48 hr. After incubation, the supernatants were collected and IL-8 production was determined by ELISA. Experiments were performed at least three times and representative results are shown. Each assay was performed in triplicate wells and data are expressed as the mean ± SEM. Significant differences between groups with and without the test specimens were seen (*P < 0·01).