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. 2003 Sep;110(1):66–72. doi: 10.1046/j.1365-2567.2003.01709.x

Figure 6.

Figure 6

Effect of mouse monoclonal antibodies to human TLR2 and TLR4 on IL-8-producing activities in PBMC stimulated with synthetic disaccharide and monosaccharide lipid A analogues. Cells were incubated with or without 10 μg/ml of mouse monoclonal antibodies to human TLR2 (TL2.1), to human TLR4 (HTA125), or mouse IgG2a at 37° for 30 min, prior to the addition of compound 506 (10 ng/ml) or various monosaccharide-type lipid A analogues (each 10 μg/ml) supplemented with 10% FBS. After 48 hr of incubation, the supernatants were collected and IL-8 production was determined by ELISA. The experimental protocols were the same as described in the legend to Figure 4. Each assay was carried out in triplicate wells and data are expressed as the mean ± SEM. Significant differences were seen as compared to the medium alone (*P < 0·01).