Figure 4.

Increased production of ROS and loss of Δψ_m in effector CTL. Naïve and P14 effector CD8⁺ T cells obtained as in Fig. 1 were untreated or treated with 5 μg/ml of plate-bound αCD3 antibody and after incubation overnight, were stained with 2 μm dihydroethidium (HE) or 40 nm DiOC₆ and analysed by flow cytometry to determine ROS production and Δψ_m, respectively. Overlay histograms of CD8-gated unstimulated (filled) and stimulated (open) cells are shown. Results are representative of two independent experiments.