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. 2003 Sep;110(1):86–94. doi: 10.1046/j.1365-2567.2003.01711.x

Figure 1.

Figure 1

Enhanced systemic and mucosal humoral responses following oral priming and systemic boosting immunizations. Mice were immunized five times intramuscularly (i.m.) with NAP and CagA purified proteins in MF59 adjuvant or five times orally with a mixture of NAP and CagA purified proteins and LTR72 adjuvant, or three times orally followed by twice i.m., or twice i.m. followed by three times orally. Sera and faecal pellets were collected 6 days after the final immunization. All immunizations were performed at 2-week intervals. Single-cell suspensions were prepared from stomach, spleen and mesenteric lymph nodes (MLN) 7 days after the final immunization for the ELISPOT assay. (a) Serum IgG titres; the results are shown as mean anti-NAP and anti-CagA serum titres from 10 mice as measured by a standard colorimetric ELISA. (b) Anti-NAP and (c) anti-CagA antibody-secreting cells (ASC). The results are shown as the mean number of anti-NAP or anti-CagA ASC from pools of 10 mice + SD of a minimum of four ELISPOT wells per group per tissue. (d) Anti-NAP IgA titres in faecal extracts. The results are shown as mean anti-NAP faecal IgA titres from 10 mice as measured by a chemiluminescence ELISA. The results are representative of three independent experiments with similar results.