Figure 4.

Induction of Th2-type cytokine-secreting cells following intranasal (i.n.) and/or intramuscular (i.m.) immunizations with CagA and NAP. Mice were immunized with five i.m. immunizations with NAP and CagA purified proteins in MF59 adjuvant (NCMF59) or five i.n. immunizations with a mixture of NAP and CagA purified proteins and LTK63 adjuvant (NCLTK63), or primed i.n. with NCLTK63 and boosted i.m. with NCMF59. All immunizations were performed at 2-week intervals. The mice were killed 7 days after the final immunization in each group. (a) The number of IL-4-secreting cells and IFN-γ-secreting cells specific for a mixture of CagA and NAP was measured by ELISPOT as described in the Materials and methods section. The results are shown as the mean number of IL-4-secreting cells from pools of 10 mice per group + SD of a minimum of four ELISPOT wells per group. The results are representative of three independent experiments with similar results. (b) Mean anti-NAP IgG1 (solid bars) or IgG2a (shaded bars) serum end-point ELISA titres + SD of 10 mice per group. The results are representative of three independent experiments with 10 mice in each group with similar results