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. 2003 Sep;110(1):18–29. doi: 10.1046/j.1365-2567.2003.01676.x

Figure 4.

Figure 4

CD4+ T cells must produce IL-10 for the generation of efferent suppressor cells. An in vitro ACAID culture system combined OVA induced ACAID APC with CD4 KO spleen cells reconstituted with CD4+ T cells from (a) IL-10 KO mice or (b) IL-4 KO mice. The putative suppressor populations were tested in a LAT assay. Positive control animals received immune cells combined with naïve cells, while negative control animals received naïve cells only. Ear swelling was measured 24 hr after intradermal injection into the ear pinna. All results are reported as mean ear swelling ± SE. (a) *P = 0·003 for positive versus negative controls. **P = 0·003 for ACAID versus positive controls. ***P = 0·003 for CD4 KO + IL-10 KO CD4 versus ACAID control. (b) *P = 0·02 for positive versus negative controls. **P = 0·02 for ACAID versus positive controls. ***P = 0·003 for CD4 KO + IL-4 KO CD4 versus positive control. Each group represents at least five animals.