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. 2003 Sep;110(1):18–29. doi: 10.1046/j.1365-2567.2003.01676.x

Figure 5.

Figure 5

IL-10 is necessary and sufficient for the generation of ACAID efferent suppressor cells. (a) In vitro spleen cell cultures combined OVA-induced ACAID APC with spleen cells from normal C57BL/6 mice cultured in the presence of anti-IL-10 antibody. The suppression of OVA-specific DTH was tested in a LAT assay. (b) In vitro ACAID cultures combined OVA-induced ACAID APC with spleen cells from CD4 KO mice in the presence of rmIL-10 cytokine. The suppression of OVA-specific DTH was tested in a LAT assay. (c) In vitro ACAID cultures combined OVA-induced ACAID APC's with spleen cells from normal C57BL/6 cultured in the presence of anti-IL-10 antibody. These cultures were tested for the ability to suppress HEL-specific immune responses. Positive control animals received immune cells combined with naïve cells, while negative control animals received naïve cells only. Ear swelling was measured 24 hr after intradermal injection into the ear pinna. All results are reported as mean ear swelling ± SE. *P = 0·03 for positive versus negative controls. **P = 0·0002 in vitro ACAID + anti-IL-10 versus negative control. (b) *P = 0·0007 for positive versus negative controls. **P = 0·001 for CD4 KO + rmIL-10 versus positive controls. (c) *P = 0·0001 for OVA positive versus OVA negative. **P = 0·0002 for OVA positive versus CD4 KO + IL-10 OVA immune. ***P = 0·03 for HEL positive versus HEL negative. ****P = 0·03 for HEL negative versus CD4 KO + IL-10 HEL immune. Each group represents at least five animals.