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. 2003 Sep;110(1):18–29. doi: 10.1046/j.1365-2567.2003.01676.x

Figure 6.

Figure 6

The CD4+ T cells needed for ACAID efferent suppressor cell production are not NKT cells. (a) In vitro ACAID cultures combined OVA-induced ACAID APC with CD4 KO spleen cells that were reconstituted with normal C57BL/6 CD4+ T cells, or CD4+ T cells depleted of NK cells. Cultures were tested for efferent suppressor cell activity in the LAT assay. (b) In vitro ACAID culture combined OVA-induced ACAID APC with normal C57BL/6 spleen cells (containing both CD4+ T cells and CD8+ T cells) or normal C57BL/6 spleen cells depleted of NK cells. The spleen cell culture was tested for suppressor cell activity using a LAT assay. Positive control animals received immune cells combined with naïve cells, while negative control animals received naïve cells only. Ear swelling was measured 24 hr after OVA injection into the ear pinna. All results are reported as mean ear swelling ± SE. (a) *P = 0·003 for positive versus negative controls. **P = 0·003 for CD4 KO + CD4 versus positive controls. ***P = 0·01 for CD4 KO + CD4+ NK− T cells versus positive control. (B) *P = 0·02 for positive versus negative control. **P = 0·008 for ACAID versus positive control. ***P = 0·0007 for positive control versus NK depleted + naïve. ****P = 0·006 for ACAID versus NK depleted + immune. Each group represents at least five animals.