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. 2003 Sep;110(1):18–29. doi: 10.1046/j.1365-2567.2003.01676.x

Figure 7.

Figure 7

CD25+ T cells are required to generate ACAID efferent suppressor cells. The in vitro ACAID cultures combined OVA-induced ACAID APC with spleen cells from CD4 KO mice reconstituted with CD4+ T cells depleted of CD25+ T cells, or T cells that were enriched for CD25+ T cells. The ACAID spleen cell cultures were tested for suppressor cell activity in a LAT assay. Positive control animals received immune cells combined with naïve cells, while negative control animals received naïve cells only. Ear swelling was measured 24 hr after OVA injection into the ear pinna. All results are reported as mean ear swelling ± SE. *P = 0·02 for positive versus negative controls. **P = 0·02 for CD4 KO + CD25+ T cells versus positive control. ***P = 0·01 for CD4 KO + CD25−CD4+ versus negative control. Each group represents at least five animals.