Table 2.
The role of IL-12 in the priming of antigen-specific wild type or IL-12 p40−/− CD4+ T cells by OVA ISCOM-loaded DC
| 4 × 104 OVA-specific T cells/well | 8 × 103 OVA-specific T cells/well | |||
|---|---|---|---|---|
| Wild type DO11.10 | DO11.10 IL-12 p40−/− | Wild type DO11.10 | DO11.10 IL-12 p40−/− | |
| Unpulsed | 2077 ± 313 | 3696 ± 633 | 1784 ± 50 | 2775 ± 554 |
| OVA ISCOMs | 81 218 ± 6516 | 53 309 ± 7061* | 18 837 ± 2713 | 12 655 ± 491* |
| OVA | 69 323 ± 5141 | 48 567 ± 5582* | 14 169 ± 1633 | 5775 ± 429* |
| OVA ISCOMs→LPS | 149 157 ± 14 365 | 125 684 ± 8207 | 46 631 ± 3558 | 34 340 ± 784* |
| OVA→LPS | 141 587 ± 6060 | 128 151 ± 344 | 39 545 ± 1900 | 16 843 ± 1762* |
*
P < 0·01 versus DO11.10 lymphocytes.
BM DC derived from BALB/c mice were pulsed with 10 µg/ml OVA ISCOMs or 1 mg/ml OVA protein for 2 hr, or with antigen and then stimulated with LPS for 24 hr, before culture with DO11.10 or DO11.10 IL-12 p40−/− lymphocyte populations containing 4 × 104 or 8 × 103 OVA specific CD4+ T cells/well. The results shown are mean c.p.m. ± 1 standard deviation for 72 hr cultures.