Figure 5.

Haemolytic assay of complement inhibition by rSh1 analogues, and the C2-binding assay. (a) Comparison of the haemolytic activities of recombinant wild-type (rSh1) and rSh1 analogues substituted at various conserved residues. Results are expressed as a percentage of wild-type haemolytic activity. (b) Western blot showing C2 that was bound to glutathione S-transferase (GST)–analogue fusions retained on glutathione–Sepharose. (c) Quantitative PhosphorImager analysis of gels shown in (b). The relative band intensity was determined by quantifying the optical density at 595 nm in each gel lane (Gel-Pro v3·1; MediaCybernetics, Carlsbad, CA).